Gapdh actin 分子量
Web我们推荐如下替代产品. Recombinant Human GAPDH protein (ab77109) Expression system: Escherichia coli. Purity: > 95% SDS-PAGE. Web常用的细胞总蛋白质内参有GAPDH和细胞骨架蛋白β-Actin或β-Tubulin等。 对于一些植物的样本,则需要特别的植物Actin蛋白作为内参。 当实验样品中只是核蛋白,而不是细胞总蛋白提取液时,可以用组蛋白H(Histone H),或者增殖细胞核抗原(PCNA)等为核内参抗体。
Gapdh actin 分子量
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WebGAPDH(甘油醛-3-磷酸脱 氢酶 ) 是参与糖 酵解的一 种关键酶,由 4个 30-40kDa 的亚 基组 成,分子量 146kDa, 检测 条 带大 约在 36kDa。 GAPDH 基因 几 乎在 所有 组 织 … Webβ-Actin Antibody 可检测 β-肌动蛋白的内源水平。由于肌动蛋白同工型之间的高序列标识,β-肌动蛋白抗体可与其他肌动蛋白同工型发生交叉反应。 物种反应性: 人, 小鼠, 大鼠, 仓鼠 , 猴, 貂 , 黑腹果蝇 , 斑马鱼 , 牛 . 基于 100% 序列同源性预测发生反应的物种:
WebGAPDH(甘油醛-3-磷酸脱氢酶)是参与 糖酵解 的一种 关键酶 ,由4个30-40kDa的 亚基 组成,分子量146kDa,检测条带大约在36kDa。. GAPDH基因几乎在所有组织中都高水平 … WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment ...
WebBackground: The use of reverse transcription-polymerase chain reaction (RT-PCR) to measure mRNA levels has led to the common use of beta-actin and GAPDH housekeeping genes as denominators for comparison of samples. Expression of these genes is assumed to remain constant, so normalising for variations in processing and signal quantitation. WebGAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" protein. It is recognized as an important enzyme involved in metabolic …
WebGAPDH分子量为146KD,检测条带大约在36kD,beta actin分子量为42KD。 一般选择内参与要检测的目的蛋白的分子量最好相差5KD以上 另外,细胞总蛋白的Western Blot的内 …
WebGAPDH Antibody (437000) in ICC/IF. Immunofluorescent analysis of GAPDH was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with GAPDH Mouse Monoclonal ... royalty free old west musicWebMar 3, 2024 · β-Actin:β-actin由375个氨基酸组成,分子量大小为42-43kDa左右。β-actin作为内参是得到了公认的,这是针对大多数组织和细胞来说的,它广泛分布于细胞浆内, … royalty free ominous musicWebNov 9, 2011 · The expression levels of this protein do not vary drastically due to cellular treatment, which is another reason the protein makes a suitable control. Novus offers a complete selection of highly … royalty free old movie footageWebMar 3, 2024 · 那么在内参的选择上面我们应该注意什么呢?. (1)根据不同的种属 ,比如哺乳动物通常选择β-actin、β-tubulin、GAPDH;植物来源实验样本,则可以选择Plant actin、Rubisco等。. (2)根据蛋白大小选择 ,一般要选择目的蛋白与内参分子量相差至少5kD以 … royalty free old time radioWebAug 5, 2015 · 在细胞处于应激、缺氧、糖代谢紊乱时最好使用actin,实验室有人做缺氧时用了GAPDH做内参,结果编辑社以此为理由给拒了,理由很简单,GAPDH是三梭酸循环途径中的一个酶.因此上述的状态可能会改变此酶的表达和活性。 ===== 那心脏组织蛋白也可以 … royalty free ominous war musicWebMar 20, 2024 · GAPDH is a housekeeping gene and used as controls in both Western blot and qPCR. GAPDH is highly conserved across species. For instance, human GAPDH with 335 amino acids shares about 70% identity with its 422 amino acids homolog from Arabidopsis thaliana. It is present in the cytosol, nucleus, perinuclear regions, and … royalty free old songsWebApr 18, 2024 · gapdhは多くの例において一貫性を示す一般的なノーマライザーです。 しかし、 いくつかの癌細胞、腫瘍抑制因子で処理した細胞、低酸素状態およびマグネシウムまたはインスリン処理した試料においては、過剰発現されることが知られています 。 royalty free one act plays for women