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Gapdh actin 分子量

WebMar 21, 2024 · Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed: 3170585, 11724794 ). Modulates the organization and assembly of the cytoskeleton (By similarity). WebGAPDH Antibody Western Blot, validation (1:1000 dilution) in brain lysate (Cat no:60004-1-Ig) This antibody offers a good detection of GAPDH on mouse cells such as macrophages and microglia through western blots. Total cell lysate (15 ug) was resolved on 4-12% Bis-Tris gels and transferred to nitrocellulose membrane.

GAPDH Monoclonal Antibody (258) (437000) - Thermo Fisher Scientific

WebFeb 8, 2024 · Actin: Tubulin: GAPDH: 中文名: 肌动蛋白: 微管蛋白: 甘油醛-3-磷酸脱氢酶: 分子量: 42kDa: 55 kDa: 36kDa: 类型: 骨架蛋白: 微管蛋白: 糖酵解反应中的一个酶: 组织特 … WebApr 27, 2013 · 分析测试百科 请教各位蛋白版高手,我最近准备做一个分子量为600多KD的大蛋白的WB实验,因该分子较大,内参选择、MARKER、转膜条件出现疑问。因为平时大家常用的主要为B-actin,B-tubulin及GAPDH,但以上这3种分子量都不大,都在30-50KD左右,且我的待测蛋白可以和actin相互作用而不能选用actin。 royalty free old paintings https://internetmarketingandcreative.com

Beta Actin and GAPDH: The Importance of Loading …

WebNational Center for Biotechnology Information Web使用 β-Actin Antibody 对 HeLa、C2C12、C6、COS 和 MvLu 细胞的提取物进行蛋白印迹分析。 显示更少 显示更多 使用 β-Actin Antibody(上图)和 Pan-Actin Antibody … Webbeta Actin、beta Tubulin、GAPDH的分子量分别是42kd,50kd,37kd,目的蛋白分子量最好能与内参蛋白分子量相差5kd以上,才能分开目的蛋白和内参,写文章做图也更美观。 … royalty free old photos

GAPDH antibody (60004-1-Ig) Proteintech - ptglab

Category:GAPDH,Tubulin,Actin选哪个做WB内参更好? - biomart.cn

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Gapdh actin 分子量

β-Actin Antibody Cell Signaling Technology

Web我们推荐如下替代产品. Recombinant Human GAPDH protein (ab77109) Expression system: Escherichia coli. Purity: > 95% SDS-PAGE. Web常用的细胞总蛋白质内参有GAPDH和细胞骨架蛋白β-Actin或β-Tubulin等。 对于一些植物的样本,则需要特别的植物Actin蛋白作为内参。 当实验样品中只是核蛋白,而不是细胞总蛋白提取液时,可以用组蛋白H(Histone H),或者增殖细胞核抗原(PCNA)等为核内参抗体。

Gapdh actin 分子量

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WebGAPDH(甘油醛-3-磷酸脱 氢酶 ) 是参与糖 酵解的一 种关键酶,由 4个 30-40kDa 的亚 基组 成,分子量 146kDa, 检测 条 带大 约在 36kDa。 GAPDH 基因 几 乎在 所有 组 织 … Webβ-Actin Antibody 可检测 β-肌动蛋白的内源水平。由于肌动蛋白同工型之间的高序列标识,β-肌动蛋白抗体可与其他肌动蛋白同工型发生交叉反应。 物种反应性: 人, 小鼠, 大鼠, 仓鼠 , 猴, 貂 , 黑腹果蝇 , 斑马鱼 , 牛 . 基于 100% 序列同源性预测发生反应的物种:

WebGAPDH(甘油醛-3-磷酸脱氢酶)是参与 糖酵解 的一种 关键酶 ,由4个30-40kDa的 亚基 组成,分子量146kDa,检测条带大约在36kDa。. GAPDH基因几乎在所有组织中都高水平 … WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment ...

WebBackground: The use of reverse transcription-polymerase chain reaction (RT-PCR) to measure mRNA levels has led to the common use of beta-actin and GAPDH housekeeping genes as denominators for comparison of samples. Expression of these genes is assumed to remain constant, so normalising for variations in processing and signal quantitation. WebGAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" protein. It is recognized as an important enzyme involved in metabolic …

WebGAPDH分子量为146KD,检测条带大约在36kD,beta actin分子量为42KD。 一般选择内参与要检测的目的蛋白的分子量最好相差5KD以上 另外,细胞总蛋白的Western Blot的内 …

WebGAPDH Antibody (437000) in ICC/IF. Immunofluorescent analysis of GAPDH was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with GAPDH Mouse Monoclonal ... royalty free old west musicWebMar 3, 2024 · β-Actin:β-actin由375个氨基酸组成,分子量大小为42-43kDa左右。β-actin作为内参是得到了公认的,这是针对大多数组织和细胞来说的,它广泛分布于细胞浆内, … royalty free ominous musicWebNov 9, 2011 · The expression levels of this protein do not vary drastically due to cellular treatment, which is another reason the protein makes a suitable control. Novus offers a complete selection of highly … royalty free old movie footageWebMar 3, 2024 · 那么在内参的选择上面我们应该注意什么呢?. (1)根据不同的种属 ,比如哺乳动物通常选择β-actin、β-tubulin、GAPDH;植物来源实验样本,则可以选择Plant actin、Rubisco等。. (2)根据蛋白大小选择 ,一般要选择目的蛋白与内参分子量相差至少5kD以 … royalty free old time radioWebAug 5, 2015 · 在细胞处于应激、缺氧、糖代谢紊乱时最好使用actin,实验室有人做缺氧时用了GAPDH做内参,结果编辑社以此为理由给拒了,理由很简单,GAPDH是三梭酸循环途径中的一个酶.因此上述的状态可能会改变此酶的表达和活性。 ===== 那心脏组织蛋白也可以 … royalty free ominous war musicWebMar 20, 2024 · GAPDH is a housekeeping gene and used as controls in both Western blot and qPCR. GAPDH is highly conserved across species. For instance, human GAPDH with 335 amino acids shares about 70% identity with its 422 amino acids homolog from Arabidopsis thaliana. It is present in the cytosol, nucleus, perinuclear regions, and … royalty free old songsWebApr 18, 2024 · gapdhは多くの例において一貫性を示す一般的なノーマライザーです。 しかし、 いくつかの癌細胞、腫瘍抑制因子で処理した細胞、低酸素状態およびマグネシウムまたはインスリン処理した試料においては、過剰発現されることが知られています 。 royalty free one act plays for women