WebNov 3, 1980 · Affinity chromatography purification of cytochrome c oxidase: Use of a yeast cytochrome c—thiol-sepharose 4B column Author links open overlay panel Kurt Bill , Robert P. Casey , Clemens Broger , Angelo Azzi WebAug 22, 2013 · Activated Thiol Sepharose 4B can be regenerated by passing one to two bed. volumes of a saturated solution (approx. 1.5 mM) of 2,2’ -dipyridyl disulphide. at pH 8.0 through the medium. 2,2’ -dipyridyl disulphide is hard to dissolve in. aqueous solutions. Make a stock solution by adding 40 mg disulphide to 50.
Activated Thiol Sepharose 4B
WebThiopropyl Sepharose 6B is suitable for immobilizing low molecular weight substances. For immobilizing high molecular weight substances, we recommend Activated Thiol Sepharose 4B (17-0640-01). Table 1. Medium characteristics. Active group: 2-pyridyl disulphide Active group concentration: 18–31 µmole 2-pyridyl disulphide/ml drained medium WebActivated Thiol Sepharose 4B Thiopropyl Sepharose 6B 12-atom hydrophilic spacer arm to couple via amino groups. As above. Coupling via primary amino groups. ... CNBr-activated Sepharose 4B CNBr-activated Sepharose 4 Fast Flow Thiopropyl Sepharose 6B (see above) 活化偶联亲和填料 mid century vanity cabinet
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WebGE Healthcare thiol sepharose 4b beads Thiol Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. WebActivated Thiol Sepharose 4B reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent … WebS3 beads are ideal replacements for commonly used activated Thiol-Sepharose 4B or Thiopropyl Sepharose 6B from GE. Product Features: High thiol binding capacity; Superior thiol reactivity; Reversible disulfide linkage; Low non-specific binding; Product Specification: Bead Matrix: 6B pegylated agarose beads; Bead Size: 50~150 microns; mid century vanity desk